Confocal leica sp5 user manual

Rmbf leica sp5 aobs spectral confocal core facilities. Choose the objective 3 in figure 1 you want to use. The leica tcssp5 upright confocal laserscanning microscope is located in room 250 west. The leica sp5 has four laser lines 405,488458, 476, 496, 514 561, and 633nm and is capable of capturing 5 channels which includes a dic brightfield detector. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. If required, switch on the mercury vapour arc lamp for detailed user guide see chapter 3. Microscope leica sp5 confocal user manual 65 pages microscope leica sp8 training manual. Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. The system was designed for confocal recor ding laser. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix a printable pdf checkin and start up. This system has five lasers generating nine lines for fluorescence excitation 405, 458, 476, 488, 496, 514, 543, 594, 633 nm.

The new leica tcs sted cw confocal microscope is the most straightforward way to user manual. Leica dm2500 upright fluorescent microscope manuals cell. The microscope is an upright dm6000 and has a motorized stage. The leica sp5 can capture images at image sizes ranging from 512x512 pixels to 8196x8196 pixels, z slices at 0. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Hexagonal packing of nuclei red by microtubules green in coeonocytic female gametophyte of ginkgo biloba. Sequential scanning using the leica tcs sp5 confocal laser scanning microscope this supplemental manual is the second section of a three part leica tcs sp5 user guide edited by donald pottle schepens 149 w dwp mar. Training manual leica tcs sp5 confocal microscope september 2010. View online or download leica sp5 confocal user manual. The leica sp5 is a pointscan confocal microscope with laser lines at 405, 458, 476, 488, 496, 514, 543, 594, and 633nm. A short tutorial about the main components of a confocal laser scanning. The leica tcs sp5 upright confocal laserscanning microscope is located in room 250 west. A better understanding of your confocal system will allow you to modify later on scan format and speed when appropriate.

The leica tcs sp5 contains many functions in its user interface that reflect its wide range of potential applications. Turning on the confocal system instructions are posted in the room 1. On the touchscreen display panel of the leica dmi8 microscope, push magnification icon 1 in figure 1 to open the control panel. Sp5 confocal microscope user manual, helene javot updated by mamta srivastava. Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. Quick operation manual for leica sp5 confocalmultiphoton microscope. Leica tcs sp5 upright confocal laserscanning microscope. These features make the leica sp5 mpconfocal a highly sensitive, flexible 2photon imaging system for thick, live tissue as well as routine confocal imaging of fixed samples. National chiao tung university, bio photonics lab, bypraveen kumar. How to use leica confocal microscope sp5 external laser. Designed by olympus, the dsu disk contains a pattern of slits that creates a virtual pinhole as the disk spins at 3,000 rpm.

Gently tilt back the arm that holds the transmitted light illumination. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy. Log into user profile press ctrl, alt and delete simultaneously enter userid and password 5. This microscope can accommodate specimens labeled with all fluorophores including ultra violet dapi. Acute and chronic live cell and living tissue imaging. Leica sp5 laser scanning confocal microscope ucsf drc. This video shows how to configure the internal laser power wavelength etc.

Leica tcs sp5 confocal laser scanning microscope user guide. Note, just because the instrument has these capabilities, does not. In addition to confocal imaging of fixed tissue samples and cells, this system is additionally equipped for. Quick operation manual for leica sp5 confocalmultiphoton. This manual is the first section of a three part leica tcs sp5 user guide. Activate the laser lines according to your fluorochromes. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. The book usually introduces a set of steps to accomplish a particular task with a distinctive heading. Improving image quality on the uacc leica sp5 confocal. Leica sp5 inverted confocal duke light microscopy core. Leica application suite x microscope software youtube. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. Start with a laser intensity of around 15 % for mean signal intensities. The confocal microscope system for highest demands in multi user facilities.

The olympus dsu disk scanning unit offers leadingedge spinning disk confocal technology. Leica sp5 laser scanning confocal microscope training. Leica dm2500 upright fluorescent microscope manuals. Only people who have had an introduction from kees straatman are allowed to operate the system. Confocal and multiphoton microscopy core school of dentistry. The sp5 is also equipped with an optional 6position motorized objective turret that holds a standard set of air and oil objectives for confocal imaging of samples. Introduction to confocal laser scanning microscopy leica.

The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Leica sp5 confocal microscope four laser uv, argon, dpss, hene. Push the eye icon 2 in figure 1 to open the port for eyepieces 2. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree. Dmi 4000 cs scanner is mo from confocal ob and eyepiece torized. Andor dragonfly spinning disk confocal microscope users manual.

Tirf, srrfstream, point localization superresolution. Pmts photomultiplier tubes or hyds gaasp hybrid detectors. If you are the last user fo the day, turn off the complete system incl. The functions not needed for a given application are disabled, however, to ensure efficiency and ease of use. The system is equipped with an inverted microscope encased with an incubator for livecell imaging. Leica sp5 upright confocal, multiphoton, flim cell. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets. Two of these detectors are very sensitive hyd sp gaaspdetectors. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. Instructions for the leica sp5 ii laser scanning confocal microscope. Leica tcssp5 upright confocal laserscanning microscope. To learn about the leica confocal software, use the book as a tutorial. It is recommended setting up a separate user id for each user set up by the system administrator.

Remove the dust cover from the microscope turn on the mercury lamp if you need to see the fluorescence through the eye pieces. Zeiss axio observer z1 manual single or tile imaging with zen. These features make the leica sp5 mp confocal a highly sensitive, flexible 2photon imaging system for thick, live tissue as well as routine confocal imaging of fixed samples. The following is the details for confocal platform as well as two microscopes. Neurobiological research has a long history not only in measurements of single cells, but also of thick brain slices. Leica microsystems sets a new standard with the integration of the leica dm6000 cfs fixed stage microscope into the leica tcs sp5 confocal platform. The leica sp5 does not use emission filters to define the wavelengths of emission collected, but.

Leica dmi6000cs inverted live cell chamber ludin cube metal halide fluorescence source motorized scanning stage tandem scanners select at software initialization conventional scanner resonant scanner 8 000 hz lasers. Ultimate optical design and stability with extreme sensitivity and superb image quality. Leica application suite x las x is the easy to use software platform for advanced life science research on leica microsystems confocal, widefield. If there is a gap, turn off the system lasers, mercury lamp, turn off the software but leave computer and hardware running. Pcic sp5 confocal microscope user manual, helene javot updated by mamta srivastava 2112017, page 116 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. An introduction from your bosssupervisor is not enough the system has 5 detectors. Laser point scan confocal with standard and highspeed resonance scanning.

Leica sp5 scanning confocal cdb equipment resource. Note, just because the instrument has these capabilities, does not mean that you need. Detection features 2 apds for confocal imaging emission separation userexchangeable beam splitting. Turning on the system 1turn on the fluorescent light source number 1. Instructions for the leica sp5 ii laser scanning confocal. The system provides the full range of scan speeds at the highest resolution. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Confocal microscopy facility schepens eye research. Log into user profile press ctrl, alt and delete simultaneously enter userid and password. Leica tcs sp5 ii laser scanning confocal microscope on an inverted microscope platform. Leicadmi6000 user manual this manual by leica microsystems gives a detailed description of the dmi6000 inverted microscope base startup and rundown procedures. This leica sp2 platform is equipped with two leica confocal microscopes. Leica tcs sp5 user guide leica sp5 sted confocal microscope. Leica sp5 inverted confocal manual duke light microscopy.

Leica tcs sp5 ii the only broadband confocal technical documentation living up to life. Leica am total internal reflection fluorescence manual. Quick guide for stepbystep protocols for the startup and closingdown of the sp5 are given at this link. Leica sp5 inverted confocal manual duke light microscopy core. From basic documentation to advanced life science research las x.

Usermanual for leica sp8 confocal universitetet i bergen. Leica tcs sp5 confocal laser scanning microscope user guide photometric 5 model upright dm 6000s. Leica sp5c spectral confocal laser scanning microscope. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Manual leica tcs sp ii university of massachusetts. Using the uacc leica sp5 confocal page 2 how big should your image be. Typing your password identifies you as a valid user for this system. Leica sp5 confocal user manual date of first issue. Leica sp5 ii confocal user guide school of dentistry. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc. Leica tcs sp5 confocal louisiana state university health. The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging.

Leica tcs sp5the newest generation of leica confocal microscopes the zmb owns an inverted leica tcs, a tsc sp2 confocal system with upright and inverted microscope stand and. Instructions for the leica sp5 ii laser scanning confocal microscope content. A high sensitivity, inverted, pointscanning confocal system. Leica sp5 general training manual sept 2010 robarts. If there is a gap, turn off the system lasers, mercury lamp, turn off the software but leave. How to use leica confocal microscope sp5 external laser part1. Be aware, when you switch between the dry and oilwater objectives, you need to push the objective icon. The leica tcs sp5 is supplied with the latest version of the licensed las af software. The confocal microscope system for highest demands in multiuser facilities. Leica application suite x las x is the one software platform for all leica microscopes.